now demonstrate such a method for three-dimensional imaging of tissue samples as large as the whole mouse brain. Tackling this problem requires a method for imaging entire brains in high enough detail to unambiguously resolve and follow axons from individual neurons across the brain. Tracing the axon of a neuron from start to end can help to explain how individual neurons and brain areas communicate signals over long distances.Ī mouse brain contains approximately 70 million neurons, and tracing the axons of many neurons within a brain is a challenging problem. These signals travel through highly branching nerve fibers called axons, which are about one hundred times thinner than a human hair, and can extend across the entire brain. Nerve cells or neurons transmit electrical impulses to each other over long distances.
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